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| Articles about Extraction |
| | | DNa Extraction | | 2008-07-14 16:09:00 | | DNA extraction is a routine procedure to collect DNA for subsequent molecular or forensic analysis. There are three basic steps in a DNA extraction:Breaking the cells open to expose the DNA within, such as by grinding or sonicating the sample.Removing membrane lipids by adding a detergent.Precipitating the DNA with an alcohol — usually ethanol or isopropanol. Since DNA is insoluble in these alcohols, it will aggregate together, giving a pellet on centrifugation. This step also removes alcohol-soluble salt.Refinements of the technique include adding a chelating agent to sequester divalent cations such as Mg2+ and Ca2+. This stops dnase enzymes from degrading the DNA.Cellular and histone proteins bound to the DNA can be removed prior to its precipitation either by adding a protease or having prior to precipitation, precipitating with sodium or ammonium acetate, or extracting with a phenol-chloroform mixture.If desired, the DNA can be resolubilized in a slightly alkaline buffer.Detecti... | | By: Biosolution | | |
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| | COPA Extraction Steps | | 2008-03-24 07:11:33 | | The below are the command steps and explanation. COPA Extraction -steps R/3 System1. KEB02. Select Datasource 1_CO_PA_CCA3. Select Field Name for Partitioning (Eg, Ccode)4. Initialise5. Select characteristics & Value Fields & Key Figures6. Select Development Class/Local Object7. Workbench Request8. Edit your Data Source to Select/Hide Fields9. Extract Checker at RSA3 & Extract BW1. Replicate Data Source2. Assign Info Source3. Transfer all Data Source elements to Info Source4. Activate Info Source5. Create Cube on Infoprovider (Copy str from Infosource)6. Go to Dimensions and create dimensions, Define & Assign7. Check & Activate8. Create Update Rules9. Insert/Modify KF and write routines (const, formula, abap)10. Activate11. Create InfoPackage for Initialization12. Maintain Infopackage13. Under Update Tab Select Initialize delta on Infopackage14. Schedule/Monitor15. Create Another InfoPackage for Delta16. Check on DELTA OptionPls r17. Ready for Delta Load LIS, CO/PA,... | | By: Free Download SAP Business Warehouse(BW) Books And | | |
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